Treatment of tumor with an extract from the shell of an aquatic animal

Abstract

A kind of marine animal with a unique evolution status, has many bioactive substances with special functions. In the present study, we investigated the antitumor effects of four different extracts derived from  shells (aqueous and methanolic extracts of burned and normal  shell powder). Aqueous and methanolic extracts of burned  shell powder were shown to be cytotoxic and inhibit the in vitro proliferation of human laryngeal carcinoma tumor cell lines (Hep-2) and human Rhabdomyosarcoma cell line (RD) and one murine mammary adenocarcinoma tumor cell line (AMN3), in the dose and time-dependent manner. Clinical and laboratory results of burnt crust extracts showed a strong antitumor effect. Inhibited % in vitro proliferation of human laryngeal carcinoma tumor cell lines (Hep-2) was 66.1%, 76.45%, and 76%  at   24hr,48 hr, and 72 hr respectively. While at human Rhabdomyosarcoma cell line (RD) Inhibited % in vitro proliferation cell lines were  67.4%, 50.6%, and 51.8% at 24 hr,48 hr, and 72 hr respectively, in murine mammary adenocarcinoma tumor cell line (AMN3), the main inhibition were 66.1 % at 250 µg/ml at 72 hr.